Qualitative Study and Antioxidant Activity Herbal Tea Bag of Moringa Leaves (Moringa oleifera Lam.) and Red Ginger (Zingiber officinale var. Rubrum)

Authors

  • Priska Ernestina Tenda Department of Pharmacy, Poltekkes Kemenkes Kupang, Kupang City, East Nusa Tenggara, Indonesia
  • Maria Hilaria Department of Pharmacy, Poltekkes Kemenkes Kupang, Kupang City, East Nusa Tenggara, Indonesia
  • Faizal R. Soeharto Department of Pharmacy, Poltekkes Kemenkes Kupang, Kupang City, East Nusa Tenggara, Indonesia
  • Mohd Nadzri Bin Mohd Najib Faculty of Pharmacy, Universiti Teknologi MARA (UiTM), Cawangan Pulau Pinang, Kampus Bertam, Malaysia

Keywords:

Antioxidants, Moringa Leaves, Red ginger, Tea bags

Abstract

Moringa (MO) leaves contain several chemical substances of high value, including vitamin C, flavonoids, phenolic compounds, carotenoids, tannins, and triterpenoids, among which one functions as an antioxidant. Previous research observed that MO leaves have already been developed into various formulations; unfortunately, these formulas could not completely obscure the aroma and taste problems that rendered MO leaves less acceptable to consumers. In this research, Moringa leaf powder (MLP) and red ginger (RG) are combined to cover the unpleasant taste and aroma of MO leaves. The purpose of this study assess the quality of three tea bag formulations, including their water content, ash content, hedonic and antioxidant activities, by quantitative tests; F0 (3 Gr of MLP), F1 (2.25 Gr of MLP, and 0.75 Gr of RG), and F2 (the amount of MLP and RG is equal to 1.5 Gr). This experimental study employs a completely randomized design, three special treatments, and three repetitions. Statistical analysis of quality test results and antioxidant activity uses One-Way ANOVA followed by a post hoc test utilizing the Least Significant Difference (LSD). The water content tests revealed that all three formulations qualified the Indonesian National Standard (SNI), ranging from 3.86% to 4.29%. Adding ginger did not influence the water content (p > 0.05). Otherwise, it increases the ash level from 8.65% to 9.98%. Based on the results of the hedonic test, the panelists preferred F0 over F1 and F2 concerning color by a score of 86%. In contrast, they preferred F2 regarding aroma and taste by a score of 82%. The addition of RG in F1 and F2 was significant to the preference level of panelists ('p <0.05). The IC50 value for the antioxidant activity test was highest for F2 at 59.31 ± 0.13 ppm. The addition of RG boosted the antioxidant activity of MO tea products considerably (sig <0.05). Consequently, developing MO tea products containing RG in F2 could be potentially produced as herbal tea beverages with antioxidant properties.      

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References

Aryanti, A., Febrina, L., Annisa, N., & Rusli, R. (2021). Antioxidant Activity of Coffee and Tea Products in Samarinda City. Jurnal Sains Dan Kesehatan, 3(3), 488–491. https://doi.org/10.25026/jsk.v3i3.510

Arumsari, K., Aminah, S. N. (2019). Total Phenol, Antioxidant Activity And Sensory Characteristic Of Kecombrang Flower, Mint Leaves, And Stevia Leaves Tea Bags. Jurnal Pangan Dan Gizi, 9(2), 128–140. https://doi.org/10.26714/jpg.9.2.2019.79-93

Asbanu, Y. W. A., Wijayati, N., & Kusumo, E. (2019). Identifikasi Senyawa Kimia Ekstrak Daun Sirsak (Annona muricata L.) dan Uji Aktivitas Antioksidannya dengan Metode DPPH (2, 2-Difenil-1-Pikrilhidrasil). Indonesian Journal of Chemical Science, 8(3), 153-160.

Aulyawati, N., Yahdi, & Suryani, N. (2021). Skrining Fitokimia Dan Aktivitas Antioksidan Ekstrak Etanol Rambut Jagung Manis (Zea Mays Ssaccharata Strurf) Menggunakan Metode DPPH. Jurnal Kimia & Pendidikan Kimia, 3(2), 132–142.

Bata, M. H., Wijaya, S., & Setiawan, H. K. (2018). Standarisasi Simplisia Kering Daun Kelor (Moringa oleifera) Dari Tiga Daerah Berbeda. Jurnal Farmasi Sains dan Terapan, 5(1), 45-52.

Cardoso, S. M., & Fazio, A. (2020). The Antioxidant Capacities of Natural Products 2019. Molecules, 25(23), 5676. https://doi.org/10.3390/molecules25235676

Casas, A. I., Nogales, C., Mucke, H. A. M., Petraina, A., Cuadrado, A., Rojo, A. I., Ghezzi, P., Jaquet, V., Augsburger, F., Dufrasne, F., Soubhye, J., Deshwal, S., Di Sante, M., Kaludercic, N., Di Lisa, F., & Schmidt, H. H. H. W. (2020). On the clinical pharmacology of reactive oxygen species. Pharmacological Reviews, 72(4), 801–828. https://doi.org/10.1124/pr.120.019422

Darna, A. R. P., M.L.M Timbuleng, E. M. L. M. T., Azzahroh, N., Khasanah, P. U., Arofah, G. E., & Kartikasari, M. N. D. (2019). PERI DALOR (Permen Jeli Daun Kelor) : Inovasi Permen Kaya Antioksidan Sebagai Solusi Kesehatan. SEMAR (Jurnal Ilmu Pengetahuan, Teknologi, Dan Seni Bagi Masyarakat), 8(1), 35–39. https://doi.org/10.20961/semar.v8i1.22062

Davies, J. M. S., Cillard, J., Friguet, B., Cadenas, E., Cadet, J., Cayce, R., Fishmann, A., Liao, D., Bulteau, A. L., Derbré, F., Rébillard, A., Burstein, S., Hirsch, E., Kloner, R. A., Jakowec, M., Petzinger, G., Sauce, D., Sennlaub, F., Limon, I., … Davies, K. J. A. (2017). The Oxygen Paradox, the French Paradox, and age-related diseases. GeroScience, 39(5–6), 499–550. https://doi.org/10.1007/s11357-017-0002-y

Dena, A., Restiani, R., & Aditiyarini, D. (2021). Peningkatan Produksi Saponin pada Kultur Kalus Ginseng Jawa (Talinum paniculatum Gaertn) dengan Penambahan Ekstrak Yeast. Sciscitatio, 2(1), 35–44. https://doi.org/10.21460/sciscitatio.2021.21.48

Dillak, H. I., Kristiani, E. B. E. K., & Kasmiyati, S. (2019). Secondary Metabolites and Anti-oxidant Activity of Ethanolic Extract of Faloak (Sterculia quadrifida). Biosaintifika Journal of Biology & Biology Education, S, 11(3), 296–303.

Dotulong, V., Wonggo, D., & Montolalu, L. A. D. Y. (2021). Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba. Chemical Science International Journal, 30(2), 23–32. https://doi.org/10.9734/csji/2021/v30i230215

Evivie, S., Ebabhamiegbebho, P., Imaren, J., & Igene, J. (2016). Evaluating the Organoleptic Properties of Soy Meatballs (BEEF) with varying Levels of Moringa oleifera Leaves Powder. Journal of Applied Sciences and Environmental Management, 19(4), 649-656. https://doi.org/10.4314/jasem.v19i4.12

Farooq, B., Koul, B., Mahant, D., & Yadav, D. (2021). Phytochemical analyses, antioxidant and anticancer activities of ethanolic leaf extracts of Moringa oleifera lam varieties. Plants, 10(11), 2348. https://doi.org/10.3390/plants10112348

Fatima, S., Masriani., & Idrus. (2020). Pengaruh Penambahan Bubuk Jahe Merah Terhadap Organoleptik Teh Celup Daun Kelor ( Moringa oleifera ). Jurnal Pengelolaan Pangan, 5(2), 42–47.https://doi.org/10.31970/pangan.v5i2.40

Fuel, M., Mesas, C., Martínez, R., Ortiz, R., Quiñonero, F., Prados, J., Porres, J. M., & Melguizo, C. (2021). Antioxidant and antiproliferative potential of ethanolic extracts from Moringa oleifera, Tropaeolum tuberosum and Annona cherimola in colorrectal cancer cells. Biomedicine and Pharmacotherapy, 143, 112248. https://doi.org/10.1016/j.biopha.2021.112248

Ghezzi, P., Jaquet, V., Marcucci, F., & Schmidt, H. H. H. W. (2017). The oxidative stress theory of disease: levels of evidence and epistemological aspects. British Journal of Pharmacology, 174(12), 1784–1796. https://doi.org/10.1111/bph.13544

Irwan, Z. (2020). Kandungan Zat Gizi Daun Kelor (Moringa Oleifera) Berdasarkan Metode Pengeringan. Jurnal Kesehatan Manarang, 6(1), 69–77. https://doi.org/10.33490/jkm.v6i1.231

Isyraqi, N. A., Rahmawati, D., & Sastyarina, Y. (2020, December). Studi Literatur: Skrining Fitokimia dan Aktivitas Farmakologi Tanaman Kelor (Moringa oleifera Lam). In Proceeding of Mulawarman Pharmaceuticals Conferences,12: 202-210. https://doi.org/10.25026/mpc.v12i1.426

Jabborova, D., Sayyed, R. Z., Azimov, A., Jabbarov, Z., Matchanov, A., Enakiev, Y., Baazeem, A., EL Sabagh, A., Danish, S., & Datta, R. (2021). Impact of mineral fertilizers on mineral nutrients in the ginger rhizome and on soil enzymes activities and soil properties. Saudi Journal of Biological Sciences, 28(9), 5268–5274. https://doi.org/10.1016/j.sjbs.2021.05.037

Khanifah, F., Puspitasari, E., & Awwaludin, S. (2021). Uji Kualitatif Flavonoid, Alkaloid, Tanin pada Kombinasi Kunyit (Curcuma Longa) dan Coklat (Theobroma cacao L). Jurnal Sains Dan Terapan Kimia, 15(1). http://dx.doi.org/10.20527/jstk.v15i1.9415

Kou, X., Li, B., Olayanju, J. B., Drake, J. M., & Chen, N. (2018). Nutraceutical or pharmacological potential of Moringa oleifera Lam. Nutrients, 10(3), 343. https://doi.org/10.3390/nu10030343

Lindawati, N. Y., & Ma’ruf, S. H. (2020). Penetapan Kadar Total Flavonoid Ekstrak Etanol Kacang Merah (Phaseolus vulgaris L.) Secara Spektrofotometri Visibel. Jurnal Ilmiah Manuntung, 6(1), 83-91. https://doi.org/10.51352/jim.v6i1.312

Malekmohammad, K., Sewell, R. D. E., & Rafieian-Kopaei, M. (2019). Antioxidants and atherosclerosis: Mechanistic aspects. Biomolecules, 9(8), 1–19. https://doi.org/10.3390/biom9080301

Manggara, A. B., & Shofi, M. (2018). Analisis Kandungan Mineral Daun Kelor (Moringa oleifera Lamk.) Menggunakan Spektrometer XRF (X-Ray Fluorescence). Akta Kimia Indonesia, 3(1), 104-111. https://doi.org/10.12962/j25493736.v3i1.3095

Mao, Q. Q., Xu, X. Y., Cao, S. Y., Gan, R. Y., Corke, H., Beta, T., & Li, H. Bin. (2019). Bioactive compounds and bioactivities of ginger (zingiber officinale roscoe). Foods, 8(6), 1–21. https://doi.org/10.3390/foods8060185

Mareta, D. T. (2019). Hedonic Test Method for Measuring Instant Pindang Seasoning Powder Preferences. Journal of Science and Applicative Technology, 3(1), 34-36. https://doi.org/10.35472/jsat.v3i1.195

Muna, L. N. (2022). Aktivitas antioksidan ekstrak air daun kelor ( Moringa oleifera ) dengan metode DPPH serta analisis kualitatif kandungan metabolit sekunder. Sasambo Journal of Pharmacy, 3(2), 91-96. https://doi.org/10.29303/sjp.v3i2.182

Ngatirah, N., & Dewi, C. W. A. (2020). Pelatihan Penggunaan Mesin Penggiling Jahe Dan Pengolahan Limbah Ampas Jahe Menjadi Bubuk Jahe. SELAPARANG Jurnal Pengabdian Masyarakat Berkemajuan, 4(1), 589-593. https://doi.org/10.31764/jpmb.v4i1.3355

Nikmah, Majid, A., & Paulus, A. Y. (2022). Identifikasi Golongan Senyawa Tanin, Flavonoid, Alkaloid dan Saponin Sebagai Senyawa Antibakteri Pada Ekstrak Daun Kelor (Moringa oleifera L.) Asal Kota Kupang. CHM-K Applied Scientific Journal, 5(1), 1–7.

Palupi, C., Nugraha, P. S. A., & Ernawaningtyas, E. (2020). Uji Mutu Sediaan Celup Daun Bunga Kertas (Bougainvillea glabra Choisy). MEDFARM: Jurnal Farmasi Dan Kesehatan, 9(1), 22–28. https://doi.org/10.48191/medfarm.v9i1.28

Pisoschi, A. M., Pop, A., Cimpeanu, C., & Predoi, G. (2016). Antioxidant capacity determination in plants and plant-derived products: A review. Oxidative Medicine and Cellular Longevity, 2016, 9130976. https://doi.org/10.1155/2016/9130976

Prawira-Atmaja, M. I., Maulana, H., Shabri, S., Riski, G. P., Fauziah, A., Harianto, S., & Rohdiana, D. (2021). Evaluasi Kesesuaian Mutu Produk Teh Dengan Persyaratan Standar Nasional Indonesia. Jurnal Standardisasi, 23(1), 43-52. https://doi.org/10.31153/js.v23i1.845

Purwoko, M. L. Y., Syamsudin, S., & Simanjuntak, P. (2022). Kombinasi Ekstrak Herba Pegagan dan Daun Kelor terhadap Kerusakan Otak dengan Metode Radial Eight-Arm Test. PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia), 19(1), 142-150. https://doi.org/10.30595/pharmacy.v19i1.13490

Rachmatiah, T., Daud, J. J., & Artanti, N. (2022). Aktivitas Antioksidan, Toksisitas, Kandungan Senyawa Fenol dan Flavonoid Total dari Daun Leilem (Clerodendrum minahassae Teijsm. & Binn). Sainstech Farma, 15(1), 35–43. https://doi.org/10.37277/sfj.v15i1.1103

Ruwandha, D., Fitriyani, D., & Iskandar, D. (2021). Uji Aktivitas Tanin Daun Mimba (Azzadirachta indica) Terhadap Bakteri Salmonella typhi. Jurnal Kimia Riset, 6(1), 77-85. https://doi.org/10.20473/jkr.v6i1.24848

Santi, I., Amirah, S., & Andriani, I. (2022). Sosialisasi Pembuatan Teh Herbal Dalam Kemasan Teh Celup Pada Kelompok Pkk Kalabbirang, Kabupaten Takalar. Dharmakarya, 11(1), 22. https://doi.org/10.24198/dharmakarya.v11i1.32667

Septiana, A. T., Handayani, I., & Winarsi, H. (2019). Aktivitas Antioksidan dan Sifat Fisikokimia Madu Temulawak (Curcuma zanthorrhiza roxb) yang Ditambah Ekstrak Jahe (Zingiber officinale Rosc). Jurnal Aplikasi Teknologi Pangan, 8(4), 155-160. https://doi.org/10.17728/jatp.4849

Setiyani, R., Fitria, A. V., Asfarida, T., & Lestari, R. D. Analisis Tipe Perilaku Konsumen dalam Membeli Teh Celup. Agricultural Socio-Economic Empowerment and Agribusiness Journal, 1(2), 49-58. https://doi.org/10.20961/agrisema.v1i2.61897

Siampa, J. P., & Jayanto, I. (2020). PKM Pemberdayaan Guru SLB Khusus Autis Permata Hati Dan SLB YPAC Manado Melalui Pelatihan Produksi Minuman Kesehatan Granul Instan Jahe Merah (Zingiber Officinale) Sebagai Upaya Peningkatan Kemandirian Ekonomi dan Terapi Supportif Siswa SLB. VIVABIO: Jurnal Pengabdian Multidisiplin, 2(2), 8. https://doi.org/10.35799/vivabio.2.2.2020.30280

Singh, V., Arulanantham, A., Parisipogula, V., Arulanantham, S., & Biswas, A. (2018). Moringa olifera: Nutrient Dense Food Source and World’s Most Useful Plant to Ensure Nutritional Security, Good Health and Eradication of Malnutrition. European Journal of Nutrition & Food Safety, 8(4), 204–214. https://doi.org/10.9734/ejnfs/2018/42468

Sinulingga, S. E., Sebayang, L. B., & Sihotang, S. (2021). Inovasi Pembuatan Teh Herbal dari Jantung Pisang dengan Tambahan Daun Stevia Sebagai Pemanis Alami. Jurnal Bios Logos, 11(2), 147-154. https://doi.org/10.35799/jbl.v11i2.35677

Siow, C. S., Chan, E. W. C., Wong, C. W., & Ng, C. W. (2022). Antioxidant and sensory evaluation of cocoa (Theobroma cacao L.) tea formulated with cocoa bean hull of different origins. Future Foods, 5(October 2021), 100108. https://doi.org/10.1016/j.fufo.2021.100108

Su, T. C., Yang, M. J., Huang, H. H., Kuo, C. C., & Chen, L. Y. (2021). Using sensory wheels to characterize consumers’ perception for authentication of taiwan specialty teas. Foods, 10(4), 1–17. https://doi.org/10.3390/foods10040836

Sucianti, A., Yusa, N. M., & Sughita, I. M. (2021). Pengaruh Suhu Pengeringan Terhadap Aktivitas Antioksidan Dan Karakteristik Teh Celup Herbal Daun Mint (Mentha piperita L.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 10(3), 378-388. https://doi.org/10.24843/itepa.2021.v10.i03.p06

Suhendy, H. (2021). Formulasi dan Evaluasi Minuman Herbal Antioksidan Jahe Merah (Zingiber officinale Rosc. var. rubrum). Jurnal Ilmiah Farmasi Farmasyifa, 4(2), 79–86. https://doi.org/10.29313/jiff.v4i2.7617

Szczurek, A. (2021). Perspectives on tannins. Biomolecules, 11(3), 1–3. https://doi.org/10.3390/biom11030442

Triandini, I.G.A.A.H., Wangiyana, I. G. A. . (2022). Mini-Review Uji Hedonik Pada Produk Teh Herbal Hutan. Jurnal Silva Samalas Journal of Forestry and Plant Science, 5(1), 12–19.

Tuapattinaya, P. M., Simal, R., & Warella, J. C. (2021). Analisis Kadar Air dan Kadar Abu Teh Berbahan Dasar Daun Lamun ( Enhalus acoroides). Jurnal Biologi Pendidikan Dan Terapan, 8(1), 16–21.

Tiyani, U., Suharti, S., & Andriani, S. (2020). Formulasi Dan Uji Organoleptik Teh Celup Daun Kersen (Muntingia Calabura L.) Untuk Memelihara Kadar Gula Darah Dan Penambahan Rimpang Jahe (Zingiber Officinale) Sebagai Penghangat Tubuh. Journal of Holistic and Health Sciences (Jurnal Ilmu Holistik dan Kesehatan), 4(1), 43-49. https://doi.org/10.51873/jhhs.v4i1.75

Wangiyana, I. G. A. S., Triandini, I. G. A. A. H., & Nugraheni, Y. M. M. A. (2021). Hedonic test of agarwood tea from Gyrinops versteegii with different leaves processing method. Jurnal Riset Industri Hasil Hutan, 13(2), 99-110. http://dx.doi.org/10.24111/jrihh.v13i2.6939

Widiastini, L. P., Karuniadi, I. G. A. M., & Tangkas, M. (2021). Senyawa Antioksidan Ekstrak Etanol Daun Kelor (Moringa Oleifera) Di Denpasar Selatan Bali. Media Kesehatan Politeknik Kesehatan Makassar, 16(1), 135-139. https://doi.org/10.32382/medkes.v16i1.2038

Widanti, Y.A., Wahyudi, H. (2019). Aktivitas Antioksidan Teh Daun Kelor (Moringa oliefera) -Rosela (Hibiscus sabdariffa L) Dengan Variasi Lama Pengeringan. Jurnal Teknologi Dan Industri Pangan, 3(2), 106-112. https://doi.org/10.33061/jitipari.v3i2.2692

Xu, D. P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J. J., & Li, H. Bin. (2017). Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. International Journal of Molecular Sciences, 18(1),96. https://doi.org/10.3390/ijms18010096

Yana, N. D., Marpaung, M. P., & Gummay, B. (2022). Analisis Parameter Spesifik dan Nonspesifik Simplisia Daun Bawang Merah (Allium cepa L.). KOVALEN: Jurnal Riset Kimia, 8(1), 45–52. https://doi.org/10.22487/kovalen.2022.v8.i1.15741

Yang, L., Liu, H., Xia, D., & Wang, S. (2020). Antioxidant properties of camphene-based thiosemicarbazones: Experimental and theoretical evaluation. Molecules, 25(5), 1192. https://doi.org/10.3390/molecules25051192

Yulianto, S. (2020). Identifikasi Alkaloid Daun Kelor (Moringa oleifera L). Jurnal Kebidanan Dan Kesehatan Tradisional, 5(1), 55–57. https://doi.org/10.37341/jkkt.v5i1.136

Yusfiani, M., Diana, A., Harahap, M., & Syakura, A. (2021). Studi Marinasi Udang Kecap Asin : Uji Hedonik. Jurnal Pengolahan Pangan, 6(1), 35–41. https://doi.org/10.31970/pangan.v6i1.48

Zela, Z., & Diah, A. W. M. (2021). Uji Aktivitas Antioksidan Ekstrak Buah Kersen (Muntingia calabura L.) Menggunakan 1,1-Difenil-2-Pikrilhidrazil. Media Eksakta, 17(2), 85–90. https://doi.org/10.22487/me.v17i2.1108

Zhang, S., Kou, X., Zhao, H., Mak, K. K., Balijepalli, M. K., & Pichika, M. R. (2022). Zingiber officinale var. rubrum: Red Ginger’s Medicinal Uses. Molecules, 27(3), 775. https://doi.org/10.3390/molecules27030775

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Published

2023-03-31

How to Cite

Tenda, P. E., Hilaria, M. ., Soeharto, F. R. ., & Najib, M. N. B. M. . (2023). Qualitative Study and Antioxidant Activity Herbal Tea Bag of Moringa Leaves (Moringa oleifera Lam.) and Red Ginger (Zingiber officinale var. Rubrum) . JURNAL INFO KESEHATAN, 21(1), 34–49. Retrieved from https://jurnal.poltekeskupang.ac.id/index.php/infokes/article/view/977

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