The Effect of Sub-Acute Inhalation Exposure to Polyethylene Micro-Nano Plastics on the Histopathological Features of the Mammary Glands in Female Wistar White Rats (Rattus Norvegicus)

Authors

  • Ihda Dian Kusuma Department of Anatomical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Laksmitha Janasti Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Riana Trinovita Sari Master Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Britania Laila Nanda Bachelor Program of Midwifery, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Hikmawan Wahyu Sulistomo Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, East Java, Indonesia
  • Nurdiana Nurdiana Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, East Java, Indonesia

DOI:

https://doi.org/10.31965/infokes.Vol22.Iss2.1534

Keywords:

Polyethylene, Cell Mamae Proliferation, Breast Cancer

Abstract

The majority of household appliances are made of plastic derived from synthetic petroleum and the result of polymerization processes. One type of plastic is Polyethylene (PE). Polyethylene (PE) contains antimony trioxide compounds that are carcinogenic in the body if ingested in excessive amounts, triggering cancer and oxidative stress, which can be observed by measuring the levels of malondialdehyde (MDA) in the body. This study aims to determine the proliferation of abnormal cells in the lactiferous ducts and mammary gland acini as well as the increase in MDA levels. The research method used is a true experimental design with a Randomized Post Test Only Group Design. This study used the mammary organs and blood of female white rats that had been exposed to PE for 28 days. The number of samples used in this study was 12 female white rats. The results showed that there were significant differences in the histopathological features and MDA levels in the mammary glands. Based on the results of the Independent T-Test on the number of cell layers in the lactiferous ducts, acini, and the number of acini (p<0.05) and the Mann-Whitney test on MDA levels (p<0.05). There were significant changes in the histopathological features and MDA levels in the mammary glands exposed to Polyethylene (PE) plastic. Exposure to micro-nano plastics of PE type at a dose of 15 mg/m3 for 28 days differed significantly in the MDA levels of mammary glands, histopathological picture of mammary glands at cell proliferation events, both in the lactiferous ducts and acini cells. In addition, exposure to PE plastic has the potential to cause health problems in the breast organs if exposed for a long time and in excess doses.

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References

Anom, I. D. K., & Lombok, J. Z. (2020). Karakterisasi Asap Cair Hasil Pirolisis Sampah Kantong Plastik sebagai Bahan Bakar Bensin. Fullerene Journal of Chemistry, 5(2), 96-101. https://doi.org/10.37033/fjc.v5i2.206

Baharuddin, A., Asran, A., & Ikhtiar, M. (2023). Spasial Analisis Mikroplastik dengan Metode FT-IR (Fourier Transform Infrared) Pada Feses Petani Kerang Hijau. Window of Health: Jurnal Kesehatan, 331-343.

Campanale, C., Massarelli, C., Savino, I., Locaputo, V., & Uricchio, V. F. (2020). A detailed review study on potential effects of microplastics and additives of concern on human health. International journal of environmental research and public health, 17(4), 1212. https://doi.org/10.3390/ijerph17041212

Cary, C. M., Seymore, T. N., Singh, D., Vayas, K. N., Goedken, M. J., Adams, S., ... & Stapleton, P. A. (2023). Single inhalation exposure to polyamide micro and nanoplastic particles impairs vascular dilation without generating pulmonary inflammation in virgin female Sprague Dawley rats. Particle and Fibre Toxicology, 20(1), 16. https://doi.org/10.1186/s12989-023-00525-x

Chang, X., Xue, Y., Li, J., Zou, L., & Tang, M. (2020). Potential health impact of environmental micro‐and nanoplastics pollution. Journal of Applied Toxicology, 40(1), 4-15. https://doi.org/10.1002/jat.3915

Chen, K., Lu, P., Beeraka, N. M., Sukocheva, O. A., Madhunapantula, S. V., Liu, J., ... & Aliev, G. (2022, August). Mitochondrial mutations and mitoepigenetics: Focus on regulation of oxidative stress-induced responses in breast cancers. In Seminars in cancer biology, 83, 556–569. https://doi.org/10.1016/j.semcancer.2020.09.012

Dhaka, V., Singh, S., Anil, A. G., Sunil Kumar Naik, T. S., Garg, S., Samuel, J., ... & Singh, J. (2022). Occurrence, toxicity and remediation of polyethylene terephthalate plastics. A review. Environmental Chemistry Letters, 20, 1777–1800. https://doi.org/10.1007/s10311-021-01384-8

Diningsih, A., & Rangkuti, N. A. (2020). Penyuluhan Pemakaian Plastik Sebagai Kemasan Makanan dan Minuman yang Aman Digunakan untuk Kesehatan di Desa Labuhan Rasoki. Jurnal Education and Development, 8(1), 17-20. https://doi.org/10.37081/ed.v8i1.1489

Enyoh, C. E., Verla, A. W., Verla, E. N., Ibe, F. C., & Amaobi, C. E. (2019). Airborne microplastics: a review study on method for analysis, occurrence, movement and risks. Environmental monitoring and assessment, 191, 668. https://doi.org/10.1007/s10661-019-7842-0

Frommlet, F., & Heinze, G. (2021). Experimental replications in animal trials. Laboratory animals, 55(1), 65-75. https://doi.org/10.1177/0023677220907617

Ghatge, S., Yang, Y., Ahn, J. H., & Hur, H. G. (2020). Biodegradation of polyethylene: a brief review. Applied Biological Chemistry, 63, 27. https://doi.org/10.1186/s13765-020-00511-3

Helm, J. S., & Rudel, R. A. (2020). Adverse outcome pathways for ionizing radiation and breast cancer involve direct and indirect DNA damage, oxidative stress, inflammation, genomic instability, and interaction with hormonal regulation of the breast. Archives of toxicology, 94(5), 1511-1549. https://doi.org/10.1007/s00204-020-02752-z

Ijaz, M. U., Ayaz, F., Mustafa, S., Ashraf, A., Albeshr, M. F., Riaz, M. N., & Mahboob, S. (2022). Toxic effect of polyethylene microplastic on testicles and ameliorative effect of luteolin in adult rats: Environmental challenge. Journal of King Saud University-Science, 34(4), 102064. https://doi.org/10.1016/j.jksus.2022.102064

Ilmiawati, I., Mahata, L. E., Aliska, G., Rustam, E., Katar, Y., Rahmatini, R., ... & Usman, E. (2022). Peningkatan Pengetahuan Masyarakat tentang Bahaya Paparan Mikroplastik dan Dampaknya bagi Kesehatan. Warta Pengabdian Andalas, 29(3), 305-311. https://doi.org/10.25077/jwa.29.3.305-311.2022

Jelic, M. D., Mandic, A. D., Maricic, S. M., & Srdjenovic, B. U. (2021). Oxidative stress and its role in cancer. Journal of cancer research and therapeutics, 17(1), 22-28. https://doi.org/10.4103/jcrt.JCRT_862_16

Karlsson, T. M., Vethaak, A. D., Almroth, B. C., Ariese, F., van Velzen, M., Hassellöv, M., & Leslie, H. A. (2017). Screening for microplastics in sediment, water, marine invertebrates and fish: Method development and microplastic accumulation. Marine pollution bulletin, 122(1-2), 403-408. https://doi.org/10.1016/j.marpolbul.2017.06.081

Kementerian Lingkungan Hidup dan Kehutanan Republik Indonesia. (2022). Capaian Kinerja Pengelolaan Sampah. Jakarta: Kementerian Lingkungan Hidup dan Kehutanan Republik Indonesia

Kementerian Kesehatan Republik Indonesia. (2022). Kanker Payudara Paling Banyak di Indonesia, Kemenkes Targetkan Pemerataan Layanan Kesehatan. Retrieved from https://sehatnegeriku.kemkes.go.id/baca/umum/20220202/1639254/kanker-payudaya-paling-banyak-di-indonesia-kemenkes-targetkan-pemerataan- layanan-kesehatan/

Kim, J., Maruthupandy, M., An, K. S., Lee, K. H., Jeon, S., Kim, J. S., & Cho, W. S. (2021). Acute and subacute repeated oral toxicity study of fragmented microplastics in Sprague-Dawley rats. Ecotoxicology and Environmental Safety, 228, 112964. https://doi.org/10.1016/j.ecoenv.2021.112964

Krisdianilo, V., Sumantri, B., & Sidabutar, R. (2021). Gambaran Sel Epitel Pada Lesi Payudara Dilaboratorium Patologi Anatomi Upt Rsud Deli Serdang Lubuk Pakam. Jurnal Farmasimed (JFM), 3(2), 100-106. https://doi.org/10.35451/jfm.v3i2.624

Maurya, R. P., Prajapat, M. K., Singh, V. P., Roy, M., Todi, R., Bosak, S., ... & Morekar, S. R. (2021). Serum malondialdehyde as a biomarker of oxidative stress in patients with primary ocular carcinoma: impact on response to chemotherapy. Clinical Ophthalmology, 871-879. https://doi.org/10.2147/OPTH.S287747

Miao, Y., Rong, M., Li, M., He, H., Zhang, L., Zhang, S., ... & Zeng, Q. (2021). Serum concentrations of organochlorine pesticides, biomarkers of oxidative stress, and risk of breast cancer. Environmental Pollution, 286, 117386. https://doi.org/10.1016/j.envpol.2021.117386

Nakai, K., & Tsuruta, D. (2021). What are reactive oxygen species, free radicals, and oxidative stress in skin diseases?. International journal of molecular sciences, 22(19), 10799. https://doi.org/10.3390/ijms221910799

Nansi, E. M., Durry, M. F., & Kairupan, C. (2015). Gambaran histopatologik payudara mencit (Mus musculus) yang diinduksi benzo (α) pyrene dan diberikan ekstrak kunyit (Curcuma longa L.). eBiomedik, 3(1), 510-515.

Park, J. H., Hong, S., Kim, O. H., Kim, C. H., Kim, J., Kim, J. W., ... & Lee, H. J. (2023). Polypropylene microplastics promote metastatic features in human breast cancer. Scientific reports, 13(1), 6252. https://doi.org/10.1038/s41598-023-33393-8

Rinjaya, T., & Mardhia, M. (2022). Pengaruh Ekstrak Etanol Daun Sirsak terhadap Histologi Tumor Payudara Tikus Putih Betina Sprague Dawley. Cermin Dunia Kedokteran, 49(9), 484-488. https://doi.org/10.55175/cdk.v49i9.292

Rudend, A. J., & Hermana, J. (2021). Kajian pembakaran sampah plastik jenis polipropilena (PP) menggunakan insinerator. Jurnal Teknik ITS, 9(2), D124-D130. https://doi.org/10.12962/j23373539.v9i2.55410

Sen, S. K., & Raut, S. (2015). Microbial degradation of low density polyethylene (LDPE): A review. Journal of Environmental Chemical Engineering, 3(1), 462-473. https://doi.org/10.1016/j.jece.2015.01.003

Shim, W. J., & Thomposon, R. C. (2015). Microplastics in the ocean. Archives of environmental contamination and toxicology, 69, 265-268. https://doi.org/10.1007/s00244-015-0216-x

Tapiory, J. G., Darjati, D., Sari, E., Narwati, N., & Ambarwati, A. (2019). Influence of Sunlight on Drinking Water in Packaging in Plastic Type of Polyethylene Terephtalate Related to Antimon Content. Jurnal Penelitian Kesehatan" SUARA FORIKES"(Journal of Health Research" Forikes Voice"), 10(1), 34-41.

Tirani, M. M., & Haghjou, M. M. (2019). Reactive oxygen species (ROS), total antioxidant capacity (AOC) and malondialdehyde (MDA) make a triangle in evaluation of zinc stress extension. JAPS: Journal of Animal & Plant Sciences, 29(4), 1100-1111.

Vianello, A., Jensen, R. L., Liu, L., & Vollertsen, J. (2019). Simulating human exposure to indoor airborne microplastics using a Breathing Thermal Manikin. Scientific reports, 9(1), 8670. https://doi.org/10.1038/s41598-019-45054-w

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Published

2024-06-30

How to Cite

Kusuma, I. D., Janasti, L., Sari, R. T., Nanda, B. L., Sulistomo, H. W., & Nurdiana, N. (2024). The Effect of Sub-Acute Inhalation Exposure to Polyethylene Micro-Nano Plastics on the Histopathological Features of the Mammary Glands in Female Wistar White Rats (Rattus Norvegicus). JURNAL INFO KESEHATAN, 22(2), 259–271. https://doi.org/10.31965/infokes.Vol22.Iss2.1534

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